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CIS

CIS

The science and practice of human immunology

The science and practice of human immunology

DISEASE-SPECIFIC FLOW CYTOMETRY TESTS OR PANELS

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ALPS Panel BTK Protein Flow
BAFF-R and TACI Expression on B cells CD18, CD11a/CD11b, and CD15s Expression (Screening for LAD)
CD40 Expression CD40L Expression
Flow Cytometry Screening for SCID (Related to IL2RG and IL7RA) DOCK8 Protein Flow
FOXP3 Expression ICOS Expression
LRBA Expression MHC Class I and Class II Expression
Perforin Screening for Perforin Deficiency/HLH due to PRF1 Mutations SAP Screening for SAP Deficiency/XLP1
WASP Screening for Wiskott-Aldrich Syndrome XIAP Screening for XIAP Deficiency/XLP2
ZAP-70 screening for SCID due to ZAP-70 deficiency  

 

ALPS Panel

ARUP
Autoimmune Lymphoproliferative Profile. Reported; % Alpha/Beta+, CD4-, CD8-, Absolute Alpha/Beta+, CD4-, CD8-, % CD5+, CD20+, Absolute CD5+, CD20+, % CD3+, HLA-DR+, Absolute CD3+, HLA-DR+. By Quantitative Flow Cytometry. Test Code: 0095505


BCH-CFCL
Immunophenotyping of γδ and CD4-CD8- αβ T cells, which reports: CD3+CD4-CD8-TCR alpha/beta T cells, CD3+TCR gamma/delta T cells. Test Code: ALPS Panel


CALGARY-CLS
The % and absolute count of CD3, CD3+4-8-TCRαβ+ DNT cells are reported as well as the % of DNT that express CD95, TCRαβ, HLA-DR and CD45R (B220). Additional antibodies to CD45R, CD25, HLA-DR CD19, and CD27 are used in an ALPS scoring system. Test Code:  ALPS


CCHMC-DIL
ALPS panel uses antibodies against CD3, CD4, CD8, CD45, alpha beta TCR, CD25, CD27, HLA-DR, CD19, CD5, CD27, and B220 to create a panel that quantifies double negative T cells, CD27+ B cells, and CD25 and HLA-DR expression on T cells to rank the likelihood of ALPS. Test Code: ALPS Panel by Flow


CHLA-DIFL
ALPS Panel Reports % CD3+CD4-CD8-αβ+, % CD3+CD4-CD8-γδ+, and %CD3+αβ+, %CD3+γδ+. Test Code: ALPS Panel Flow


MAYO-ML
Uses antibodies against CD3, CD4, CD8, CD45, alpha beta TCR, and B220 to quantify Double Negative T Cells, analyze B220 expression. Test Code: ALPS; #82449


MCW-CIRL
Autoimmune Lymphoproliferative Syndrome Panel includes CD3, CD4, CD8, CD16, CD56, CD19, CD14, CD15, CD11b, CD45, CD21, CD27, IgD, TCRαβ, B220, HLA-DR, CD25. Test Code: AILYMP


MONTREAL-SJH
CD3+CD4−CD8−/TCRαβ double negative T cells enumeration. Test Code: CD4(-)CD8(-)/ TCRαβ


NJH-ADL
Reports percentages of DNTs using a CD3/CD48/TCRαβ panel. Test Code: ALPS


NSUH
T-lymphocyte TCR subset uses CD3, CD4, CD8, TCRα/β, TCR γ/δ, B220, CD20, CD5, and CD45.  Percentages are reported. Test Code: ALPS

 

BTK Protein Flow

ARUP
Bruton Tyrosine Kinase (BTK) Protein Expression by Flow Cytometry (Qualitative Flow Cytometry). Test Code: 2012002


CALGARY-CLS
Bruton Tyrosine Kinase (BTK) Protein Expression by Flow Cytometry in B cells and monocytes. Test Code: BTK


MAYO-ML
Analyzes BTK expression in B cells and monocytes. Test Code: BTK; #89011


MCW-CIRL
Analyzes BTK expression in B cells and monocytes. Test Code: BTK

 

BAFF-R and TACI Expression on B cells

MAYO-ML
Reports the percent of CD19+ B cells expressing TACI and BAFF-R. Test Code: CVID; #87993


NJH-ADL
Reports BAFF-R qualitatively as present/absent and includes commentary comparing the expression level to that of a date matched normal control. Test Code: BAFFR

 

CD18, CD11a/CD11b, and CD15s Expression (Screening for LAD)

ARUP
Leukocyte Adhesion Deficiency Panel. Panel measures CD11b, CD15, and CD18 on neutrophils. By Semi-Quantitative Flow Cytometry. Test Code: 2004359


BCH-CFCL
Leukocyte Adhesion Deficiency, which reports: CD18+ cells, CD15s+ cells. Test Code: LAD Panel


CALGARY-CLS
Measures expression of CD18, CD11a, CD11b, CD11c (Type I LAD) and CD15s (Type II LAD) on the surface of granulocytes. Test Code: LAD


CCHMC-DIL
Measures CD18 and CD11b expression on neutrophils. Test Code: Neutrophil Adhesion Mrkrs: CD18/11b


CHLA-DIFL
Leukocyte Adhesion Deficiency Type-1.  Measures expression of CD18, CD11a, CD11b and CD15s on the surface of granulocytes. Test Code: Leukocyte Adhesion Deficiency Type 1


MAYO-ML
Measures CD18, CD11a, and CD11b on neutrophils. Provides % data for control and patient in interpretive report for abnormal results. Test Code: LAD1; #81155


MCW-CIRL
PID2 test measures CD18 and CD11b on neutrophils. Test Code: PID2


NJH-ADL
Qualitative analysis of CD11a,b,c and CD18 on neutrophils. Test Code: ADHM


NSUH
LAD panel uses CD11a, CD11b, CD11c, and CD18.  Percentages are reported. Test Code: LAD


QUEST
Leukocyte Adhesion Deficiency (LAD) Evaluation (CD15s, CD18). Flow cytometry panel measures CD11b, CD15, and CD18 expression on neutrophils. Test Code: 19815

 

CD40 Expression

CALGARY-CLS
CD40 expression on B cells is assessed.  Testing includes the CD40L (CD154) assay. Test Code: LINK


MAYO-ML
The cell surface expression of CD40 by B cells (CD19+) is determined and expressed as being present or absent. Test Code: CD40; #89009


MCW-CIRL
Measures CD40 expression on B cells and CD40L expression on activated CD4 cells. Test Code: HIGM


NJH-ADL
CD40 expression on CD19+ B cells. Qualitative interpretation – present/absent is reported.  Test Code: CD40M

 

CD40L Expression

BCH-CFCL
Immunophenotyping of CD40 Ligand (CD154)- positive T lymphocytes, which reports: CD3+CD8-CD154+, CD3+CD8-CD69+. Test Code: CD40 Ligand assay


CALGARY-CLS
CD40L (CD154) expression is assessed on CD3+4+8- cells after PMA/Ionomycin activation.  Testing includes assessment of CD40 on B cells. Test Code: LINK


CCHMC-DIL
Evaluation of CD40L Upregulation (and ICOS Upregulation). Test Code: CD40L / ICOS


MAYO-ML
Evaluation of CD40L Upregulation, also includes assessment of binding to receptor using soluble CD40-muIg. Test Code: XHIM; #82964


MCW-CIRL
Measures CD40 expression on B cells and CD40L expression on activated CD4 cells. Test Code: HIGM


NJH-ADL
Analyzes CD40L expression in whole blood, gating on CD3+/CD8- T cells. Reports qualitative result – absent/present. Test Code: CD40L

 

Flow Cytometry Screening for SCID (Related to IL2RG and IL7RA)

CALGARY-CLS
Assesses both CD132 and CD127 on peripheral blood lymphocytes: Test Code: CD127/CD132


CCHMC-DIL
Analyzes CD132 and CD127 expression on T cells, NK cells, and B cells. Code: CD127/CD132

 

DOCK8 Protein Flow

CALGARY-CLS
Assesses DOCK8 protein expression in peripheral blood lymphocyte subsets (T, B, NK). Test Code: DOCK8


MONTREAL-SJH
DOCK8 detection by flow cytometry. Test Code: Marqueurs intracytoplasmique: Autres and indicate yourself

 

FOXP3 Expression

CALGARY-CLS
Reports the % and absolute count of Tregs determined by CD3+ CD4+ lowCD127+ and CD25+. Test Code: TREG


CCHMC-DIL
Quantifies the percent of T regs (CD25+ CD127lo) as a percentage of the CD4+ T cells, and quantifies the percentage of T regs, which express FOXP3. Test Code: FoxP3


CHLA-DIFL
Regulatory T-Cell (T Reg). Percent of CD4+ CD25+ CD127dim is reported. Test Code:


MCW-CIRL
Measures Foxp3+, CD127, CD25 expression on CD4 cells. Test Code: TREG


MONTREAL-SJH
Percentages of regulatory T cells: CD4+ CD25high CD127- Foxp3+ by flow cytometry. Test Code: FOXp3


NJH-ADL
National Jewish Health using a 4-color panel, CD3/4/CD25/ FoxP3, gating on CD25highFoxP3+ cells. Reports percentages. Test Code: FOXP3


NSUH
Regulatory T-Lymphocytes uses CD4, CD25, CD127, and FoxP3.  Percentages are reported. Test Code: FoxP3

 

ICOS Expression

CALGARY-CLS
Reports ICOS (CD278) expression on CD3+4+8- cells pre and post stimulation with PMA/Ionomycin. Test Code: ICOS


CCHMC-DIL
The CD40L Assay includes measurement of ICOS before and after activation of T Cells. Test Code: CD40L / ICOS


NJH-ADL
Analyzes ICOS expression in whole blood, gating on CD3+/CD8- T cells. Reports qualitative result – absent/present. Test Code: ICOS

 

LRBA Expression

CALGARY-CLS
Analyzes lipopolysaccharide Responsive Beige-like Anchor (LRBA) protein expression in CD3+ T cells. Percentage and median fluorescent intensity compared to a simultaneously run normal control is reported. Test code: LRBA

 

MHC Class I and Class II Expression

CCHMC-DIL
Class I (HLA-A, B and C) and Class II (HLA-DR) epitope expression on peripheral blood lymphocytes and monocytes are performed by multiparameter flow cytometry. Test Code: MHC Class I & II


CHLA-DIFL
Bare lymphocyte syndrome - MHC Class II deficiency assay. Test Code: T Cell Extended


MCW-CIRL
Primary Immunodeficiency 2 panel includes CD3, CD4, CD8, TCRαβ, CD69, CD45RA, CD45RO, CD16, CD56; CD19, CD20, CD27, CD5, CD14, CD15, CD11b, CD18, CD45, HLA-DR, HLA-ABC. Test Code: PID2


MONTREAL-SJH
Test of HLA class I expression. Test Code: HLA class I

Test of HLA class II expression on B lymphocytes (CD19+) and monocytes (CD14+). Test Code: HLA class II

 

Perforin Screening for Perforin Deficiency/HLH due to PRF1 Mutations

CALGARY-CLS
Assesses perforin and granzyme B expression in NK cells and CD8+ T cells. Test Code: HLH


CCHMC-DIL
Analyzes perforin expression in CD8+ T cells, NK cells. Also measures and reports granzyme B expression. Test Code: Perforin/Granzyme B


MAYO-ML
See NKSP test under NK cell subsets.  Measures perforin and Granzyme B expression in cytotoxic NK cells.  Test Code: NKSP;  #60615


MCW-CIRL
Perforin, Granzyme expression in CD56 and CD8 cells. Test Code: PERGRA


MONTREAL-SJH
Perforin detection by flow cytometry. Test Code: Perforine

 

SAP Screening for SAP Deficiency/XLP1

CALGARY-CLS
Measures SAP expression in T and NK cells. Test Code: XLP1


CCHMC-DIL
Analyzes SAP expression in T cells and NK cells. Test Code: SAP (XLP1)


MCW-CIRL
Assay measures SAP and XIAP expression in peripheral blood lymphocytes. Test Code: XLP


MONTREAL-SJH
SAP detection by flow cytometry. Test Code: Marqueurs intracytoplasmique: Autres and indicate yourself

 

WASP Screening for Wiskott-Aldrich Syndrome

CALGARY-CLS
Measures WAS in peripheral blood lymphocytes and monocytes and reports percentage and median fluorescent channel compared to a simultaneously run normal control.  Test Code:  WASP


CCHMC-DIL
Regular Panel (WASP) determines WASP expression in peripheral blood lymphocytes and reports expression as a ratio to control cells. Test Code: WASP

WAS Transplant Monitor Panel measures WASP expression in T cells, NK cells, B cells, monocytes, and granulocytes. Test Code: WASP Transplant Monitor

 

XIAP Screening for XIAP Deficiency/XLP2

CALGARY-CLS
Assay measures both SAP and XIAP expression in lymphocyte subsets: Test Code: XLP2


CCHMC-DIL
Assay measures XIAP expression in T cells, NK cells, and B cells. Test Code: XIAP (XLP2)


MCW-CIRL
Assay measures SAP and XIAP expression in peripheral blood lymphocytes. Test Code: XLP


MONTREAL-SJH
XIAP detection by flow cytometry. Test Code: Marqueurs intracytoplasmique: Autres and indicate yourself

 

ZAP-70 screening for SCID due to ZAP-70 deficiency

CALGARY-CLS
ZAP-70 protein expression is measured in T and NK cell subsets.Test Code: ZAP70 SCID


CCHMC-DIL
Assay measures ZAP-70 expression in T cells and NK cells. Test Code: ZAP-70.