ALPS Panel
ARUP
Autoimmune Lymphoproliferative Profile. Reported; % Alpha/Beta+, CD4-, CD8-, Absolute Alpha/Beta+, CD4-, CD8-, % CD5+, CD20+, Absolute CD5+, CD20+, % CD3+, HLA-DR+, Absolute CD3+, HLA-DR+. By Quantitative Flow Cytometry. Test Code: 0095505
BCH-CFCL
Immunophenotyping of γδ and CD4-CD8- αβ T cells, which reports: CD3+CD4-CD8-TCR alpha/beta T cells, CD3+TCR gamma/delta T cells. Test Code: ALPS Panel
CALGARY-CLS
The % and absolute count of CD3, CD3+4-8-TCRαβ+ DNT cells are reported as well as the % of DNT that express CD95, TCRαβ, HLA-DR and CD45R (B220). Additional antibodies to CD45R, CD25, HLA-DR CD19, and CD27 are used in an ALPS scoring system. Test Code: ALPS
CCHMC-DIL
ALPS panel uses antibodies against CD3, CD4, CD8, CD45, alpha beta TCR, CD25, CD27, HLA-DR, CD19, CD5, CD27, and B220 to create a panel that quantifies double negative T cells, CD27+ B cells, and CD25 and HLA-DR expression on T cells to rank the likelihood of ALPS. Test Code: ALPS Panel by Flow
CHLA-DIFL
ALPS Panel Reports % CD3+CD4-CD8-αβ+, % CD3+CD4-CD8-γδ+, and %CD3+αβ+, %CD3+γδ+. Test Code: ALPS Panel Flow
MAYO-ML
Uses antibodies against CD3, CD4, CD8, CD45, alpha beta TCR, and B220 to quantify Double Negative T Cells, analyze B220 expression. Test Code: ALPS; #82449
MCW-CIRL
Autoimmune Lymphoproliferative Syndrome Panel includes CD3, CD4, CD8, CD16, CD56, CD19, CD14, CD15, CD11b, CD45, CD21, CD27, IgD, TCRαβ, B220, HLA-DR, CD25. Test Code: AILYMP
MONTREAL-SJH
CD3+CD4−CD8−/TCRαβ double negative T cells enumeration. Test Code: CD4(-)CD8(-)/ TCRαβ
NJH-ADL
Reports percentages of DNTs using a CD3/CD48/TCRαβ panel. Test Code: ALPS
NSUH
T-lymphocyte TCR subset uses CD3, CD4, CD8, TCRα/β, TCR γ/δ, B220, CD20, CD5, and CD45. Percentages are reported. Test Code: ALPS
BTK Protein Flow
ARUP
Bruton Tyrosine Kinase (BTK) Protein Expression by Flow Cytometry (Qualitative Flow Cytometry). Test Code: 2012002
CALGARY-CLS
Bruton Tyrosine Kinase (BTK) Protein Expression by Flow Cytometry in B cells and monocytes. Test Code: BTK
MAYO-ML
Analyzes BTK expression in B cells and monocytes. Test Code: BTK; #89011
MCW-CIRL
Analyzes BTK expression in B cells and monocytes. Test Code: BTK
BAFF-R and TACI Expression on B cells
MAYO-ML
Reports the percent of CD19+ B cells expressing TACI and BAFF-R. Test Code: CVID; #87993
NJH-ADL
Reports BAFF-R qualitatively as present/absent and includes commentary comparing the expression level to that of a date matched normal control. Test Code: BAFFR
CD18, CD11a/CD11b, and CD15s Expression (Screening for LAD)
ARUP
Leukocyte Adhesion Deficiency Panel. Panel measures CD11b, CD15, and CD18 on neutrophils. By Semi-Quantitative Flow Cytometry. Test Code: 2004359
BCH-CFCL
Leukocyte Adhesion Deficiency, which reports: CD18+ cells, CD15s+ cells. Test Code: LAD Panel
CALGARY-CLS
Measures expression of CD18, CD11a, CD11b, CD11c (Type I LAD) and CD15s (Type II LAD) on the surface of granulocytes. Test Code: LAD
CCHMC-DIL
Measures CD18 and CD11b expression on neutrophils. Test Code: Neutrophil Adhesion Mrkrs: CD18/11b
CHLA-DIFL
Leukocyte Adhesion Deficiency Type-1. Measures expression of CD18, CD11a, CD11b and CD15s on the surface of granulocytes. Test Code: Leukocyte Adhesion Deficiency Type 1
MAYO-ML
Measures CD18, CD11a, and CD11b on neutrophils. Provides % data for control and patient in interpretive report for abnormal results. Test Code: LAD1; #81155
MCW-CIRL
PID2 test measures CD18 and CD11b on neutrophils. Test Code: PID2
NJH-ADL
Qualitative analysis of CD11a,b,c and CD18 on neutrophils. Test Code: ADHM
NSUH
LAD panel uses CD11a, CD11b, CD11c, and CD18. Percentages are reported. Test Code: LAD
QUEST
Leukocyte Adhesion Deficiency (LAD) Evaluation (CD15s, CD18). Flow cytometry panel measures CD11b, CD15, and CD18 expression on neutrophils. Test Code: 19815
CD40 Expression
CALGARY-CLS
CD40 expression on B cells is assessed. Testing includes the CD40L (CD154) assay. Test Code: LINK
MAYO-ML
The cell surface expression of CD40 by B cells (CD19+) is determined and expressed as being present or absent. Test Code: CD40; #89009
MCW-CIRL
Measures CD40 expression on B cells and CD40L expression on activated CD4 cells. Test Code: HIGM
NJH-ADL
CD40 expression on CD19+ B cells. Qualitative interpretation – present/absent is reported. Test Code: CD40M
CD40L Expression
BCH-CFCL
Immunophenotyping of CD40 Ligand (CD154)- positive T lymphocytes, which reports: CD3+CD8-CD154+, CD3+CD8-CD69+. Test Code: CD40 Ligand assay
CALGARY-CLS
CD40L (CD154) expression is assessed on CD3+4+8- cells after PMA/Ionomycin activation. Testing includes assessment of CD40 on B cells. Test Code: LINK
CCHMC-DIL
Evaluation of CD40L Upregulation (and ICOS Upregulation). Test Code: CD40L / ICOS
MAYO-ML
Evaluation of CD40L Upregulation, also includes assessment of binding to receptor using soluble CD40-muIg. Test Code: XHIM; #82964
MCW-CIRL
Measures CD40 expression on B cells and CD40L expression on activated CD4 cells. Test Code: HIGM
NJH-ADL
Analyzes CD40L expression in whole blood, gating on CD3+/CD8- T cells. Reports qualitative result – absent/present. Test Code: CD40L
Flow Cytometry Screening for SCID (Related to IL2RG and IL7RA)
CALGARY-CLS
Assesses both CD132 and CD127 on peripheral blood lymphocytes: Test Code: CD127/CD132
CCHMC-DIL
Analyzes CD132 and CD127 expression on T cells, NK cells, and B cells. Code: CD127/CD132
DOCK8 Protein Flow
CALGARY-CLS
Assesses DOCK8 protein expression in peripheral blood lymphocyte subsets (T, B, NK). Test Code: DOCK8
MONTREAL-SJH
DOCK8 detection by flow cytometry. Test Code: Marqueurs intracytoplasmique: Autres and indicate yourself
FOXP3 Expression
CALGARY-CLS
Reports the % and absolute count of Tregs determined by CD3+ CD4+ lowCD127+ and CD25+. Test Code: TREG
CCHMC-DIL
Quantifies the percent of T regs (CD25+ CD127lo) as a percentage of the CD4+ T cells, and quantifies the percentage of T regs, which express FOXP3. Test Code: FoxP3
CHLA-DIFL
Regulatory T-Cell (T Reg). Percent of CD4+ CD25+ CD127dim is reported. Test Code:
MCW-CIRL
Measures Foxp3+, CD127, CD25 expression on CD4 cells. Test Code: TREG
MONTREAL-SJH
Percentages of regulatory T cells: CD4+ CD25high CD127- Foxp3+ by flow cytometry. Test Code: FOXp3
NJH-ADL
National Jewish Health using a 4-color panel, CD3/4/CD25/ FoxP3, gating on CD25highFoxP3+ cells. Reports percentages. Test Code: FOXP3
NSUH
Regulatory T-Lymphocytes uses CD4, CD25, CD127, and FoxP3. Percentages are reported. Test Code: FoxP3
ICOS Expression
CALGARY-CLS
Reports ICOS (CD278) expression on CD3+4+8- cells pre and post stimulation with PMA/Ionomycin. Test Code: ICOS
CCHMC-DIL
The CD40L Assay includes measurement of ICOS before and after activation of T Cells. Test Code: CD40L / ICOS
NJH-ADL
Analyzes ICOS expression in whole blood, gating on CD3+/CD8- T cells. Reports qualitative result – absent/present. Test Code: ICOS
LRBA Expression
CALGARY-CLS
Analyzes lipopolysaccharide Responsive Beige-like Anchor (LRBA) protein expression in CD3+ T cells. Percentage and median fluorescent intensity compared to a simultaneously run normal control is reported. Test code: LRBA
MHC Class I and Class II Expression
CCHMC-DIL
Class I (HLA-A, B and C) and Class II (HLA-DR) epitope expression on peripheral blood lymphocytes and monocytes are performed by multiparameter flow cytometry. Test Code: MHC Class I & II
CHLA-DIFL
Bare lymphocyte syndrome - MHC Class II deficiency assay. Test Code: T Cell Extended
MCW-CIRL
Primary Immunodeficiency 2 panel includes CD3, CD4, CD8, TCRαβ, CD69, CD45RA, CD45RO, CD16, CD56; CD19, CD20, CD27, CD5, CD14, CD15, CD11b, CD18, CD45, HLA-DR, HLA-ABC. Test Code: PID2
MONTREAL-SJH
Test of HLA class I expression. Test Code: HLA class I
Test of HLA class II expression on B lymphocytes (CD19+) and monocytes (CD14+). Test Code: HLA class II
Perforin Screening for Perforin Deficiency/HLH due to PRF1 Mutations
CALGARY-CLS
Assesses perforin and granzyme B expression in NK cells and CD8+ T cells. Test Code: HLH
CCHMC-DIL
Analyzes perforin expression in CD8+ T cells, NK cells. Also measures and reports granzyme B expression. Test Code: Perforin/Granzyme B
MAYO-ML
See NKSP test under NK cell subsets. Measures perforin and Granzyme B expression in cytotoxic NK cells. Test Code: NKSP; #60615
MCW-CIRL
Perforin, Granzyme expression in CD56 and CD8 cells. Test Code: PERGRA
MONTREAL-SJH
Perforin detection by flow cytometry. Test Code: Perforine
SAP Screening for SAP Deficiency/XLP1
CALGARY-CLS
Measures SAP expression in T and NK cells. Test Code: XLP1
CCHMC-DIL
Analyzes SAP expression in T cells and NK cells. Test Code: SAP (XLP1)
MCW-CIRL
Assay measures SAP and XIAP expression in peripheral blood lymphocytes. Test Code: XLP
MONTREAL-SJH
SAP detection by flow cytometry. Test Code: Marqueurs intracytoplasmique: Autres and indicate yourself
WASP Screening for Wiskott-Aldrich Syndrome
CALGARY-CLS
Measures WAS in peripheral blood lymphocytes and monocytes and reports percentage and median fluorescent channel compared to a simultaneously run normal control. Test Code: WASP
CCHMC-DIL
Regular Panel (WASP) determines WASP expression in peripheral blood lymphocytes and reports expression as a ratio to control cells. Test Code: WASP
WAS Transplant Monitor Panel measures WASP expression in T cells, NK cells, B cells, monocytes, and granulocytes. Test Code: WASP Transplant Monitor
XIAP Screening for XIAP Deficiency/XLP2
CALGARY-CLS
Assay measures both SAP and XIAP expression in lymphocyte subsets: Test Code: XLP2
CCHMC-DIL
Assay measures XIAP expression in T cells, NK cells, and B cells. Test Code: XIAP (XLP2)
MCW-CIRL
Assay measures SAP and XIAP expression in peripheral blood lymphocytes. Test Code: XLP
MONTREAL-SJH
XIAP detection by flow cytometry. Test Code: Marqueurs intracytoplasmique: Autres and indicate yourself
ZAP-70 screening for SCID due to ZAP-70 deficiency
CALGARY-CLS
ZAP-70 protein expression is measured in T and NK cell subsets.Test Code: ZAP70 SCID
CCHMC-DIL
Assay measures ZAP-70 expression in T cells and NK cells. Test Code: ZAP-70.